Identification of Il-1α Gene from Bovine Peripheral Blood Mononuclear Cells by Polymerase Chain Reaction

Interleukin-1 (IL-1) play its role in inflammation, fever and release of acute phase proteins by acting on major organs of the body. IL1α gene along with other cytokines compels Th1 and Th17 inflammatory responses. The present study is aimed at standardization of polymerase chain reaction for amplification of IL-1α gene from bovine peripheral blood mononuclear cells. Blood samples were collected in containers with 0.1% Ethylenediaminetetraacetic acid (EDTA) anticoagulant (Sigma, USA). Peripheral blood mononuclear cells (PBMCs) were isolated using commercially available Histopaque®-1077. Extracted total ribose nucleic acid (RNA) was utilized in reverse transcription to prepare complementary deoxyribose nucleic acid (cDNA). IL-1α gene specific primers were used to amlify partial nucleotide sequence of IL-1α. The cDNA was employed in polymerase chain reaction (PCR). The resulting IL-1α DNA product (124 bp) was identified on agar gel electrophoresis and documented.